Inbred maize line PH05G

ABSTRACT

An inbred maize line, designated PH05G, the plants and seeds of inbred maize line PH05G, methods for producing a maize plant produced by crossing the inbred line PH05G with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PH05G with another maize line or plant.

FIELD OF THE INVENTION

This invention is in the field of maize breeding, specifically relatingto an inbred maize line designated PH05G.

BACKGROUND OF THE INVENTION

The goal of plant breeding is to combine in a single variety or hybridvarious desirable traits. For field crops, these traits may includeresistance to diseases and insects, tolerance to heat and drought,reducing the time to crop maturity, greater yield, and better agronomicquality. With mechanical harvesting of many crops, uniformity of plantcharacteristics such as germination and stand establishment, growthrate, maturity, and plant and ear height, is important.

Field crops are bred through techniques that take advantage of theplant's method of pollination. A plant is self-pollinated if pollen fromone flower is transferred to the same or another flower of the sameplant. A plant is cross-pollinated if the pollen comes from a flower ona different plant.

Plants that have been self-pollinated and selected for type for manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny. A cross between twodifferent homozygous lines produces a uniform population of hybridplants that may be heterozygous for many gene loci. A cross of twoplants each heterozygous at a number of gene loci will produce apopulation of hybrid plants that differ genetically and will not beuniform.

Maize (Zea mays L.), often referred to as corn in the United States, canbe bred by both self-pollination and cross-pollination techniques. Maizehas separate male and female flowers on the same plant, located on thetassel and the ear, respectively. Natural pollination occurs in maizewhen wind blows pollen from the tassels to the silks that protrude fromthe tops of the ears.

A reliable method of controlling male fertility in plants offers theopportunity for improved plant breeding. This is especially true fordevelopment of maize hybrids, which relies upon some sort of malesterility system. There are several options for controlling malefertility available to breeders, such as: manual or mechanicalemasculation (or detasseling), cytoplasmic male sterility, genetic malesterility, gametocides and the like.

Hybrid maize seed is typically produced by a male sterility systemincorporating manual or mechanical detasseling. Alternate strips of twoinbreds of maize are planted in a field, and the pollen-bearing tasselsare removed from one of the inbreds (female). Providing that there issufficient isolation from sources of foreign maize pollen, the ears ofthe detasseled inbred will be fertilized only from the other inbred(male), and the resulting seed is therefore hybrid and will form hybridplants.

The laborious, and occasionally unreliable, detasseling process can beavoided by using cytoplasmic male-sterile (CMS) inbreds. Plants of a CMSinbred are male sterile as a result of factors resulting from thecytoplasmic, as opposed to the nuclear, genome. Thus, thischaracteristic is inherited exclusively through the female parent inmaize plants, since only the female provides cytoplasm to the fertilizedseed. CMS plants are fertilized with pollen from another inbred that isnot male-sterile. Pollen from the second inbred may or may notcontribute genes that make the hybrid plants male-fertile. Seed fromdetasseled fertile maize and CMS produced seed of the same hybrid can beblended to insure that adequate pollen loads are available forfertilization when the hybrid plants are grown.

There are several methods of conferring genetic male sterilityavailable, such as multiple mutant genes at separate locations withinthe genome that confer male sterility, as disclosed in U.S. Pat. Nos.4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations asdescribed by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. Theseand all patents referred to are incorporated by reference. In additionto these methods, Albertsen et al., of Pioneer Hi-Bred, U.S. patentapplication Ser. No. 07/848,433, have developed a system of nuclear malesterility which includes: identifying a gene which is critical to malefertility; silencing this native gene which is critical to malefertility; removing the native promoter from the essential malefertility gene and replacing it with an inducible promoter; insertingthis genetically engineered gene back into the plant; and thus creatinga plant that is male sterile because the inducible promoter is not "on"resulting in the male fertility gene not being transcribed. Fertility isrestored by inducing, or turning "on" the promoter, which in turn allowsthe gene that confers male fertility to be transcribed.

There are many other methods of conferring genetic male sterility in theart, each with its own benefits and drawbacks. These methods use avariety of approaches such as delivering into the plant a gene encodinga cytotoxic substance associated with a male tissue specific promoter oran antisense system in which a gene critical to fertility is identifiedand an antisense to that gene is inserted in the plant (see:Fabinjanski, et al. EPO 89/3010153.8 publication no. 329,308 and PCTapplication PCT/CA90/00037 published as WO 90/08828).

Another system useful in controlling male sterility makes use ofgametocides. Gametocides are not a genetic system, but rather a topicalapplication of chemicals. These chemicals affect cells that are criticalto male fertility. The application of these chemicals affects fertilityin the plants only for the growing season in which the gametocide isapplied (see Carison, Glenn R., U.S. Pat. No. 4,936,904). Application ofthe gametocide, timing of the application and genotype specificity oftenlimit the usefulness of the approach.

The use of male sterile inbreds is but one factor in the production ofmaize hybrids. The development of maize hybrids requires, in general,the development of homozygous inbred lines, the crossing of these lines,and the evaluation of the crosses. Pedigree breeding and recurrentselection breeding methods are used to develop inbred lines frombreeding populations. Breeding programs combine the genetic backgroundsfrom two or more inbred lines or various other germplasm sources intobreeding pools from which new inbred lines are developed by selfing andselection of desired phenotypes. The new inbreds are crossed with otherinbred lines and the hybrids from these crosses are evaluated todetermine which of those have commercial potential. Plant breeding andhybrid development are expensive and time consuming processes.

Pedigree breeding starts with the crossing of two genotypes, each ofwhich may have one or more desirable characteristics that is lacking inthe other or which complements the other. If the two original parents donot provide all the desired characteristics, other sources can beincluded in the breeding population. In the pedigree method, superiorplants are selfed and selected in successive generations. In thesucceeding generations the heterozygous condition gives way tohomogeneous lines as a result of self-pollination and selection.Typically in the pedigree method of breeding five or more generations ofselfing and selection is practiced: F₁ →F₂ ; F₃ →F₄ ; F₄ →F₅, etc.

Recurrent selection breeding, backcrossing for example, can be used toimprove an inbred line. Backcrossing can be used to transfer a specificdesirable trait from one inbred or source to an inbred that lacks thattrait. This can be accomplished, for example, by first crossing asuperior inbred (recurrent parent) to a donor inbred (non-recurrentparent), that carries the appropriate gene(s) for the trait in question.The progeny of this cross is then mated back to the superior recurrentparent followed by selection in the resultant progeny for the desiredtrait to be transferred from the non-recurrent parent. After five ormore backcross generations with selection for the desired trait, theprogeny will be heterozygous for loci controlling the characteristicbeing transferred, but will be like the superior parent for most oralmost all other genes. The last backcross generation is then selfed togive pure breeding progeny for the gene(s) being transferred.

A single cross maize hybrid results from the cross of two inbred lines,each of which has a genotype that complements the genotype of the other.The hybrid progeny of the first generation is designated F₁. In thedevelopment of commercial hybrids only the F₁ hybrid plants are sought.Preferred F₁ hybrids are more vigorous than their inbred parents. Thishybrid vigor, or heterosis, can be manifested in many polygenic traits,including increased vegetative growth and increased yield.

The development of a maize hybrid involves three steps: (1) theselection of plants from various germplasm pools for initial breedingcrosses; (2) the selfing of the selected plants from the breedingcrosses for several generations to produce a series of inbred lines,which, although different from each other, breed true and are highlyuniform; and (3) crossing the selected inbred lines with differentinbred lines to produce the hybrid progeny (F₁). During the inbreedingprocess in maize, the vigor of the lines decreases. Vigor is restoredwhen two different inbred lines are crossed to produce the hybridprogeny (F₁). An important consequence of the homozygosity andhomogeneity of the inbred lines is that the hybrid between a definedpair of inbreds will always be the same. Once the inbreds that give asuperior hybrid have been identified, the hybrid seed can be reproducedindefinitely as long as the homogeneity of the inbred parents ismaintained.

A single cross hybrid is produced when two inbred lines are crossed toproduce the F₁ progeny. A double cross hybrid is produced from fourinbred lines crossed in pairs (A×B and C×D) and then the two F₁ hybridsare crossed again (A×B)×(C×D). Much of the hybrid vigor exhibited by F₁hybrids is lost in the next generation (F₂). Consequently, seed fromhybrids is not used for planting stock.

Hybrid seed production requires elimination or inactivation of pollenproduced by the female parent. Incomplete removal or inactivation of thepollen provides the potential for self pollination. This inadvertentlyself pollinated seed may be unintentionally harvested and packaged withhybrid seed.

Once the seed is planted, it is possible to identify and select theseself pollinated plants. These self pollinated plants will be geneticallyequivalent to the female inbred line used to produce the hybrid.

Typically these self pollinated plants can be identified and selecteddue to their decreased vigor. Female selfs are identified by their lessvigorous appearance for vegetative and/or reproductive characteristics,including shorter plant height, small ear size, ear and kernel shape,cob color, or other characteristics.

Identification of these self pollinated lines can also be accomplishedthrough molecular marker analyses. See, "The Identification of FemaleSelfs in Hybrid Maize: A Comparison Using Electrophoresis andMorphology", Smith, J. S. C. and Wych, R. D., Seed Science andTechnology 14, pp. 1-8 (1995), the disclosure of which is expresslyincorporated herein by reference. Through these technologies, thehomozygosity of the self pollinated line can be verified by analyzingallelic composition at various loci along the genome. Those methodsallow for rapid identification of the invention disclosed herein. Seealso, "Identification of Atypical Plants in Hybrid Maize Seed byPostcontrol and Electrophoresis" Sarca, V. et al., Probleme de GeneticaTeoritica si Aplicata Vol. 20 (1) p. 29-42. As is readily apparent toone skilled in the art, the foregoing are only two of the various waysby which the inbred can be obtained by those looking to use thegermplasm. Other means are available, and the above examples areillustrative only.

Maize is an important and valuable field crop. Thus, a continuing goalof plant breeders is to develop high-yielding maize hybrids that areagronomically sound based on stable inbred lines. The reasons for thisgoal are obvious: to maximize the amount of grain produced with theinputs used and minimize susceptibility of the crop to pests andenvironmental stresses. To accomplish this goal, the maize breeder mustselect and develop superior inbred parental lines for producing hybrids.This requires identification and selection of genetically uniqueindividuals that occur in a segregating population. The segregatingpopulation is the result of a combination of crossover events plus theindependent assortment of specific combinations of alleles at many geneloci that results in specific genotypes. The probability of selectingany one individual with a specific genotype from a breeding cross isinfinitesimal due to the large number of segregating genes and theunlimited recombinations of these genes, some of which may be closelylinked. However, the genetic variation among individual progeny of abreeding cross allows for the identification of rare and valuable newgenotypes. These new genotypes are neither predictable nor incrementalin value, but rather the result of manifested genetic variation combinedwith selection methods, environments and the actions of the breeder.

Thus, even if the entire genotypes of the parents of the breeding crosswere characterized and a desired genotype known, only a few, if any,individuals having the desired genotype may be found in a largesegregating F₂ population. Typically, however, neither the genotypes ofthe breeding cross parents nor the desired genotype to be selected isknown in any detail. In addition, it is not known how the desiredgenotype would react with the environment. This genotype by environmentinteraction is an important, yet unpredictable, factor in plantbreeding. A breeder of ordinary skill in the art cannot predict thegenotype, how that genotype will interact with various climaticconditions or the resulting phenotypes of the developing lines, exceptperhaps in a very broad and general fashion. A breeder of ordinary skillin the art would also be unable to recreate the same line twice from thevery same original parents as the breeder is unable to direct how thegenomes combine or how they will interact with the environmentalconditions. This unpredictability results in the expenditure of largeamounts of research resources in the development of a superior new maizeinbred line.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel inbred maize line,designated PH05G. This invention thus relates to the seeds of inbredmaize line PH05G, to the plants of inbred maize line PH05G, and tomethods for producing a maize plant produced by crossing the inbred linePH05G with itself or another maize line. This invention further relatesto hybrid maize seeds and plants produced by crossing the inbred linePH05G with another maize line.

Definitions

In the description and examples that follow, a number of terms are usedherein. In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided. NOTE: ABS is in absolute termsand % MN is percent of the mean for the experiments in which the inbredor hybrid was grown. These designators will follow the descriptors todenote how the values are to be interpreted. Below are the descriptorsused in the data tables included herein.

ANT ROT=ANTHRACNOSE STALK ROT (Colletotrichum graminicola). A 1 to 9visual rating indicating the resistance to Anthracnose Stalk Rot. Ahigher score indicates a higher resistance.

BAR PLT=BARREN PLANTS. The percent of plants per plot that were notbarren (lack ears).

BRT STK=BRITTLE STALKS. This is a measure of the stalk breakage near thetime of pollination, and is an indication of whether a hybrid or inbredwould snap or break near the time of flowering under severe winds. Dataare presented as percentage of plants that did not snap.

BU ACR=YIELD (BUSHELS/ACRE). Yield of the grain at harvest in bushelsper acre adjusted to 15.5% moisture.

CLD TST=COLD TEST. The percent of plants that germinate under cold testconditions.

CLN=CORN LETHAL NECROSIS. Synergistic interaction of maize chloroticmottle virus (MCMV) in combination with either maize dwarf mosaic virus(MDMV-A or MDMV-B) or wheat streak mosaic virus (WSMV). A 1 to 9 visualrating indicating the resistance to Corn Lethal Necrosis. A higher scoreindicates a higher resistance.

COM RST=COMMON RUST (Puccinia sorghi). A 1to 9 visual rating indicatingthe resistance to Common Rust. A higher score indicates a higherresistance.

D/D=DRYDOWN. This represents the relative rate at which a hybrid willreach acceptable harvest moisture compared to other hybrids on a 1-9rating scale. A high score indicates a hybrid that dries relatively fastwhile a low score indicates a hybrid that dries slowly.

DIP ERS=DIPLODIA EAR MOLD SCORES (Diplodia maydis and Diplodiamacrospora). A 1 to 9 visual rating indicating the resistance toDiplodia Ear Mold. A higher score indicates a higher resistance.

DRP EAR=DROPPED EARS. A measure of the number of dropped ears per plotand represents the percentage of plants that did not drop ears prior toharvest.

D/T=DROUGHT TOLERANCE. This represents a 1-9 rating for droughttolerance, and is based on data obtained under stress conditions. A highscore indicates good drought tolerance and a low score indicates poordrought tolerance.

EAR HT=EAR HEIGHT. The ear height is a measure from the ground to thehighest placed developed ear node attachment and is measured in inches.

EAR MLD=General Ear Mold. Visual rating (1-9 score) where a "1" is verysusceptible and a "9" is very resistant. This is based on overall ratingfor ear mold of mature ears without determining the specific moldorganism, and may not be predictive for a specific ear mold.

EAR SZ=EAR SIZE. A 1 to 9 visual rating of ear size. The higher therating the larger the ear size.

ECB 1LF=EUROPEAN CORN BORER FIRST GENERATION LEAF FEEDING (Ostrinianubilalis). A 1 to 9 visual rating indicating the resistance topreflowering leaf feeding by first generation European Corn Borer. Ahigher score indicates a higher resistance.

ECB 2IT=EUROPEAN CORN BORER SECOND GENERATION INCHES OF TUNNELING(Ostinia nubilalis). Average inches of tunneling per plant in the stalk.

ECB 2SC=EUROPEAN CORN BORER SECOND GENERATION (Ostfinia nubilalis). A 1to 9 visual rating indicating post flowering degree of stalk breakageand other evidence of feeding by European Corn Borer, Second Generation.A higher score indicates a higher resistance.

ECB DPE=EUROPEAN CORN BORER DROPPED EARS (Ostrinia nubilalis). Droppedears due to European Corn Borer. Percentage of plants that did not dropears under second generation corn borer infestation.

EST CNT=EARLY STAND COUNT. This is a measure of the stand establishmentin the spring and represents the number of plants that emerge on perplot basis for the inbred or hybrid.

EYE SPT=Eye Spot (Kabatiella zeae or Aureobasidium zeae). A 1 to 9visual rating indicating the resistance to Eye Spot. A higher scoreindicates a higher resistance.

FUS ERS=FUSARIUM EAR ROT SCORE (Fusarium moniliforne or Fusariumsubglutinans). A 1 to 9 visual rating indicating the resistance toFusarium ear rot. A higher score indicates a higher resistance.

GDU=Growing Degree Units. Using the Barger Heat Unit Theory, whichassumes that maize growth occurs in the temperature range 50° F.-86° F.and that temperatures outside this range slow down growth; the maximumdaily heat unit accumulation is 36 and the minimum daily heat unitaccumulation is 0. The seasonal accumulation of GDU is a major factor indetermining maturity zones.

GDU SHD=GDU TO SHED. The number of growing degree units (GDUs) or heatunits required for an inbred line or hybrid to have approximately 50percent of the plants shedding pollen and is measured from the time ofplanting. Growing degree units are calculated by the Barger Method,where the heat units for a 24-hour period are: ##EQU1##

The highest maximum temperature used is 86° F. and the lowest minimumtemperature used is 50° F. For each inbred or hybrid it takes a certainnumber of GDUs to reach various stages of plant development.

GDU SLK=GDU TO SILK. The number of growing degree units required for aninbred line or hybrid to have approximately 50 percent of the plantswith silk emergence from time of planting. Growing degree units arecalculated by the Barger Method as given in GDU SHD definition.

GIB ERS=GIBBERELLA EAR ROT (PINK MOLD) (Gibberella zeae). A 1 to 9visual rating indicating the resistance to Gibberella Ear Rot. A higherscore indicates a higher resistance.

GLF SPT=Gray Leaf Spot (Cercospora zeae-maydis). A 1 to 9 visual ratingindicating the resistance to Gray Leaf Spot. A higher score indicates ahigher resistance.

GOS WLT=Goss' Wilt (Corynebacterium nebraskense). A 1 to 9 visual ratingindicating the resistance to Goss' Wilt. A higher score indicates ahigher resistance.

GRN APP=GRAIN APPEARANCE. This is a 1 to 9 rating for the generalappearance of the shelled grain as it is harvested based on such factorsas the color of harvested grain, any mold on the grain, and any crackedgrain. High scores indicate good grain quality.

H/POP=YIELD AT HIGH DENSITY. Yield ability at relatively high plantdensities on 1-9 relative rating system with a higher number indicatingthe hybrid responds well to high plant densities for yield relative toother hybrids. A 1, 5, and 9 would represent very poor, average, andvery good yield response, respectively, to increased plant density.

HC BLT=HELMINTHOSPORIUM CARBONUM LEAF BLIGHT (Helminthosporiumcarbonum). A 1 to 9 visual rating indicating the resistance toHelminthosporum infection. A higher score indicates a higher resistance.

HD SMT=HEAD SMUT (Sphacelotheca reiliana). This score indicates thepercentage of plants not infected.

INC D/A=GROSS INCOME (DOLLARS PER ACRE). Relative income per acreassuming drying costs of two cents per point above 15.5 percent harvestmoisture and current market price per bushel.

INCOME/ACRE. Income advantage of hybrid to be patented over other hybridon per acre basis.

INC ADV=GROSS INCOME ADVANTAGE. GROSS INCOME advantage of variety #1over variety #2.

KSZ DCD=KERNEL SIZE DISCARD. The percent of discard seed; calculated asthe sum of discarded tip kernels and extra large kernels.

L/POP=YIELD AT LOW DENSITY. Yield ability at relatively low plantdensities on a 1-9 relative system with a higher number indicating thehybrid responds well to low plant densities for yield relative to otherhybrids. A 1, 5, and 9 would represent very poor, average, and very goodyield response, respectively, to low plant density.

MDM CPX=MAIZE DWARF MOSAIC COMPLEX (MDMV=Maize Dwarf Mosaic Virus andMCDV=Maize Chlorotic Dwarf Virus). A 1 to 9 visual rating indicating theresistance to Maize Dwarf Mosaic Complex. A higher score indicates ahigher resistance.

MST=HARVEST MOISTURE. The moisture is the actual percentage moisture ofthe grain at harvest.

MST ADV=MOISTURE ADVANTAGE. The moisture advantage of variety #1 overvariety #2 as calculated by: MOISTURE of variety #2-MOISTURE of variety#1=MOISTURE ADVANTAGE of variety #1.

NLF BLT=Northern Leaf Blight (Helminthospodum turcicum or Exserohilumturcicum). A 1 to 9 visual rating indicating the resistance to NorthernLeaf Blight. A higher score indicates a higher resistance.

PLT HT=PLANT HEIGHT. This is a measure of the height of the plant fromthe ground to the tip of the tassel in inches.

POL SC=POLLEN SCORE. A 1 to 9 visual rating indicating the amount ofpollen shed. The higher the score the more pollen shed.

POL WT=POLLEN WEIGHT. This is calculated by dry weight of tasselscollected as shedding commences minus dry weight from similar tasselsharvested after shedding is complete.

It should be understood that the inbred can, through routinemanipulation of cytoplasmic or other factors, be produced in amale-sterile form. Such embodiments are also contemplated within thescope of the present claims.

POP K/A=PLANT POPULATIONS. Measured as 1000s per acre.

POP ADV=PLANT POPULATION ADVANTAGE. The plant population advantage ofvariety #1 over variety #2 as calculated by PLANT POPULATION of variety#2-PLANT POPULATION of variety #1=PLANT POPULATION ADVANTAGE of variety#1.

PRM=PREDICTED RELATIVE MATURITY. This trait, predicted relativematurity, is based on the harvest moisture of the grain. The relativematurity rating is based on a known set of checks and utilizes standardlinear regression analyses and is also referred to as the ComparativeRelative Maturity Rating System that is similar to the MinnesotaRelative Maturity Rating System.

PRM SHD=A relative measure of the growing degree units (GDU) required toreach 50% pollen shed. Relative values are predicted values from thelinear regression of observed GDU's on relative maturity of commercialchecks.

RT LDG=ROOT LODGING. Root lodging is the percentage of plants that donot root lodge; plants that lean from the vertical axis at anapproximately 300° angle or greater would be counted as root lodged.

RTL ADV=ROOT LODGING ADVANTAGE. The root lodging advantage of variety #1over variety #2.

SCT GRN=SCATTER GRAIN. A 1 to 9 visual rating indicating the amount ofscatter grain (lack of pollination or kernel abortion) on the ear. Thehigher the score the less scatter grain.

SDG VGR=SEEDLING VIGOR. This is the visual rating (1 to 9) of the amountof vegetative growth after emergence at the seedling stage(approximately five leaves). A higher score indicates better vigor.

SEL IND=SELECTION INDEX. The selection index gives a single measure ofthe hybrid's worth based on information for up to five traits. A maizebreeder may utilize his or her own set of traits for the selectionindex. One of the traits that is almost always included is yield. Theselection index data presented in the tables represent the mean valueaveraged across testing stations.

SI/WTA=SILAGE WEIGHT (TONS PER ACRE). Yield of silage at harvest in tonsper acre adjusted to 65% moisture.

SLF BLT=SOUTHERN LEAF BLIGHT (Helminthospofium maydis or Bipoladsmaydis). A 1 to 9 visual rating indicating the resistance to SouthernLeaf Blight. A higher score indicates a higher resistance.

SOU RST=SOUTHERN RUST (Puccinia polysora). A 1 to 9 visual ratingindicating the resistance to Southern Rust. A higher score indicates ahigher resistance.

STA GRN=STAY GREEN. Stay green is the measure of plant health near thetime of black layer formation (physiological maturity). A high scoreindicates better late- season plant health.

STD ADV=STALK STANDING ADVANTAGE. The advantage of variety #1 overvariety #2 for the trait STK CNT.

STK CNT=NUMBER OF PLANTS. This is the final stand or number of plantsper plot.

STK LDG=STALK LODGING. This is the percentage of plants that did notstalk lodge (stalk breakage) as measured by either natural lodging orpushing the stalks and determining the percentage of plants that breakbelow the ear.

STW WLT=Stewart's Wilt (Erwinia stewartii). A 1 to 9 visual ratingindicating the resistance to Stewart's Wilt. A higher score indicates ahigher resistance.

TAS BLS=TASSEL BLAST. A 1 to 9 visual rating was used to measure thedegree of blasting (necrosis due to heat stress) of the tassel at thetime of flowering. A 1 would indicate a very high level of blasting attime of flowering, while a 9 would have no tassel blasting.

TAS SZ=TASSEL SIZE. A 1 to 9 visual rating was used to indicate therelative size of the tassel. The higher the rating the larger thetassel.

TAS WT=TASSEL WEIGHT. This is the average weight of a tassel (grams)just prior to pollen shed.

TEX EAR=EAR TEXTURE. A 1 to 9 visual rating was used to indicate therelative hardness (smoothness of crown) of mature grain. A 1 would bevery soft (extreme dent) while a 9 would be very hard (flinty or verysmooth crown).

TILLER=TILLERS. A count of the number of tillers per plot that couldpossibly shed pollen was taken. Data are given as a percentage oftillers: number of tillers per plot divided by number of plants perplot.

TST WT=TEST WEIGHT (UNADJUSTED). The measure of the weight of the grainin pounds for a given volume (bushel).

TST WTA=TEST WEIGHT ADJUSTED. The measure of the weight of the grain inpounds for a given volume (bushel) adjusted for 15.5 percent moisture.

TSW ADV=TEST WEIGHT ADVANTAGE. The test weight advantage of variety #1over variety #2.

WIN M %=PERCENT MOISTURE WINS.

WIN Y %=PERCENT YIELD WINS.

YLD=YIELD. It is the same as BU ACR ABS.

YLD ADV=YIELD ADVANTAGE. The yield advantage of variety #1 over variety#2 as calculated by: YIELD of variety #1-YIELD variety #2=yieldadvantage of variety #1.

YLD SC=YIELD SCORE. A 1 to 9 visual rating was used to give a relativerating for yield based on plot ear piles. The higher the rating thegreater visual yield appearance.

DETAILED DESCRIPTION OF THE INVENTION

Inbred maize lines are typically developed for use in the production ofhybrid maize lines. Inbred maize lines need to be highly homogeneous,homozygous and reproducible to be useful as parents of commercialhybrids. There are many analytical methods available to determine thehomozygotic and phenotypic stability of these inbred lines.

The oldest and most traditional method of analysis is the observation ofphenotypic traits. The data is usually collected in field experimentsover the life of the maize plants to be examined. Phenotypiccharacteristics most often observed are for traits associated with plantmorphology, ear and kernel morphology, insect and disease resistance,maturity, and yield.

In addition to phenotypic observations, the genotype of a plant can alsobe examined. There are many laboratory-based techniques available forthe analysis, comparison and characterization of plant genotype; amongthese are Isozyme Electrophoresis, Restriction Fragment LengthPolymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs),Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Amplified Fragment Length Polymorphisms (AFLPs), and Simple SequenceRepeats (SSRs) which are also referred to as Microsatellites.

The most widely used of these laboratory techniques are IsozymeElectrophoresis and RFLPs as discussed in Lee, M., "Inbred Lines ofMaize and Their Molecular Markers," The Maize Handbook,(Springer-Verlag, New York, Inc. 1994, at 423-432) incorporated hereinby reference. Isozyme Electrophoresis is a useful tool in determininggenetic composition, although it has relatively low number of availablemarkers and the low number of allelic variants among maize inbreds.RFLPs have the advantage of revealing an exceptionally high degree ofallelic variation in maize and the number of available markers is almostlimitless.

Maize RFLP linkage maps have been rapidly constructed and widelyimplemented in genetic studies. One such study is described inBoppenmaier, et al., "Comparisons among strains of inbreds for RFLPs",Maize Genetics Cooperative Newsletter, 65:1991, pg. 90, is incorporatedherein by reference. This study used 101 RFLP markers to analyze thepatterns of 2 to 3 different deposits each of five different inbredlines. The inbred lines had been selfed from 9 to 12 times before beingadopted into 2 to 3 different breeding programs. It was results fromthese 2 to 3 different breeding programs that supplied the differentdeposits for analysis. These five lines were maintained in the separatebreeding programs by selfing or sibbing and rogueing off-type plants foran additional one to eight generations. After the RFLP analysis wascompleted, it was determined the five lines showed 0-2% residualheterozygosity. Although this was a relatively small study, it can beseen using RFLPs that the lines had been highly homozygous prior to theseparate strain maintenance.

Inbred maize line PH05G is a yellow, dent maize inbred that is suited asa male for producing first generation F1 maize hybrids. Inbred maizeline PH05G is best adapted to the Northwest, Northcentral, Northeast andCentral Corn Belt regions of the United States and can be used toproduce hybrids from approximately 99-106 relative maturity based on theComparative Relative Maturity Rating System for harvest moisture ofgrain. Inbred maize line PH05G demonstrates strong stalks, good roots,tall plants with high ear placement and very good resistance to Goss'Wilt and Head Smut. In hybrid combination, PH05G demonstrates excellentyields, excellent stay green, strong stalks and roots, rapid drydown andproduces high grain silage tonnage. For its area of adaptation PH05Ghybrids demonstrate rapid drydown, excellent yields, very good stalks,roots and stay green, and produces large plants with a resulting highgrain silage tonnage.

The inbred has shown uniformity and stability within the limits ofenvironmental influence for all the traits as described in the VarietyDescription Information (Table 1) that follows. The inbred has beenself-pollinated and ear-rowed a sufficient number of generations withcareful attention paid to uniformity of plant type to ensure thehomozygosity and phenotypic stability necessary to use in commercialproduction. The line has been increased both by hand and in isolatedfields with continued observation for uniformity. No variant traits havebeen observed or are expected in PH05G.

Inbred maize line PH05G, being substantially homozygous, can bereproduced by planting seeds of the line, growing the resulting maizeplants under self-pollinating or sib-pollinating conditions withadequate isolation, and harvesting the resulting seed, using techniquesfamiliar to the agricultural arts.

                                      TABLE 1                                     __________________________________________________________________________    VARIETY DESCRIPTION INFORMATION                                               VARIETY = PH05G                                                               __________________________________________________________________________     1.                                                                             TYPE: (describe intermediate types in Comments section):                    2   1 = Sweet 2 = Dent 3 = Flint 4 = Flour 5 = Pop 6 = Ornamental               MATURITY:                                                                   DAYS   HEAT UNITS                                                             070    1,382.8                                                                              From emergence to 50% of plants in silk                         068    1,338.5                                                                              From emergence to 50% of plants in pollen                       006    0,138.0                                                                              From 10% to 90% pollen shed                                     069    1,271.8                                                                              From 50% silk to harvest at 25% moisture                          PLANT:                                                                                                           Standard                                                                           Sample                                                                   Deviation                                                                          Size                                0,204.8                                                                             cm Plant Height (to tassel tip)                                                                              5.25  4                                  0,081.8                                                                             cm Ear Height (to base of top ear node)                                                                      2.63  4                                  0,016.1                                                                             cm Length of Top Ear Internode 1.42 15                                  1        Average Number of Tillers   0.00  4                                  1.3      Average Number of Ears per Stalk                                                                          0.50  4                                  3.0      Anthocyanin of Brace Roots: 1 = Absent 2 = Faint 3 = Moderate 4               = Dark                                                                 LEAF:                                                                                                            Standard                                                                           Sample                                                                   Deviation                                                                          Size                                008.8 cm Width of Ear Node Leaf       0.40                                                                              20                                  070.8 cm Length of Ear Node Leaf      5.81                                                                              20                                  05.9     Number of leaves above top ear                                                                             0.42                                                                              15                                  051.7    Degrees Leaf Angle (measure from 2nd leaf above                                                           21.59                                                                               3                                           ear at anthesis to stalk above leaf)                                 03       Leaf Color                                                                         Dark Green                                                                          (Munsell code)                                                                         5GY34                                            1.0      Leaf Sheath Pubescence (Rate on scale from 1 = none to 9 = like               peach fuzz)                                                          6.5      Marginal Waves (Rate on scale from 1 = none to 9 = many)             6.3      Longitudinal Creases (Rate on scale from 1 = none to 9 = many)         TASSEL:                                                                                                          Standard                                                                           Sample                                                                   Deviation                                                                          Size                                02.6     Number of Primary Lateral Branches                                                                         1.40                                                                              20                                  057.3    Branch Angle from Central Spike                                                                           21.87                                                                               4                                  53.5  cm Tassel Length (from top leaf collar to tassel tip)                                                        3.16  4                                  5.8      Pollen Shed (rate on scale from 0 = male sterile to 9 = heavy                 shed)                                                                01       Anther Color                                                                         Light Green                                                                           (Munsell code)                                                                          2.5GY86                                     01       Glume Color                                                                          Light Green                                                                           (Munsell code)                                                                          5GY76                                       1.0      Bar Glumes (Glume Bands): 1 = Absent 2 = Present                     17       Peduncle Length (cm. from top leaf to basal branches)                6a.                                                                             EAR (Unhusked Data):                                                        11       Silk Color (3 days after emergence)                                                               Pink   (Munsell code)                                                                       7.5R74                              2       Fresh Husk Color (25 days after 50% silking)                                                      Medium Green                                                                         (Munsell code)                                                                       5GY66                              21       Dry Husk Color (65 days after 50% silking)                                                        Buff   (Munsell code)                                                                       10YR92                              2       Position of Ear at Dry Husk Stage: 1 = Upright 2 = Horizontal 3               = Pendant                        Horizontal                           5       Husk Tightness (Rate of Scale from 1 = very loose to 9 = very                 tight)                                                                2       Husk Extension (at harvest): 1 = Short (ears exposed) 2 = Medium              (<8 cm)                                                                       3 = Long (8-10 cm beyond ear tip) 4 = Very Long (>10                                                           Medium                              6b.                                                                             EAR (Husked Ear Data):                                                                                           Standard                                                                           Sample                                                                   Deviation                                                                          Size                                 17   cm Ear Length                   0.50                                                                              20                                   39   mm Ear Diameter at mid-point    2.38                                                                              20                                  104   gm Ear Weight                  21.00                                                                              20                                   13      Number of Kernel Rows        0.96                                                                              20                                   2       Kernel Rows: 1 = Indistinct 2 = Distinct                                                                            Distinct                        1       Row Alignment: 1 = Straight 2 = Slightly Curved 3                                                                   Straight                        19   cm Shank Length                 3.08                                                                              20                                   2       Ear Taper: 1 = Slight 2 = Average 3 = Extreme                                                                       Average                          KERNEL (Dried):                                                                                                  Standard                                                                           Sample                                                                   Deviation                                                                          Size                                11    mm Kernel Length                0.57                                                                              20                                   8    mm Kernel Width                 0.35                                                                              20                                   5    mm Kernel Thickness             0.00                                                                              20                                  35       % Round Kernels (Shape Grade)                                                                             17.36                                                                               4                                   1       Aleurone Color Pattern: 1 = Homozygous 2 = Segregating                                                              Homozygous                      7       Aluerone Color                                                                           Yellow           (Munsell code)                                                                          10YR712                         7       Hard Endosperm Color                                                                     Yellow           (Munsell code)                                                                          10YR814                         3       Endosperm Type:                                                                          Normal Starch                                                      1 = Sweet (Su1) 2 = Extra Sweet (sh2) 3 = Normal Starch                       4 = High Amylose Starch 5 = Waxy Starch 6 = High Protein                      7 = High Lysine 8 = Super Sweet (se) 9 = High Oil                             10 = Other.sub.----------                                            29    gm Weight per 100 Kernels (unsized sample)                                                                    2.22                                                                               4                                    COB:                                                                                                             Standard                                                                           Sample                                                                   Deviation                                                                          Size                                22    mm Cob Diameter at mid-point   0.84 20                                  14       Cob Color                                                                          Red                    (Munsell ccde)                                                                          10R46                           2       Cob Strength 1 = Weak 2 = Strong                                       DISEASE RESISTANCE                                                                        (Rate from 1 (most susceptible) to 9 (most resistant);                        leave blank                                                                   if not tested; leave Race or Strain Options blank if                          polygenic):                                                     A. Leaf Blights, Wilts, and Local Infection Diseases                                Anthracnose Leaf Blight ( Colletotrichum graminicola)                   5     Common Rust (Puccinia sorghi)                                                 Common Smut (Ustilago                                                   8     Eyespot (Kabatiella zeae) maydis)                                       7     Goss's Wilt (Clavibacter michiganense spp. nebraskense)                 4     Gray Leaf Spot (Cercospora zeae-maydis)                                       Helminthosporium Leaf Spot (Bipolaris zeicola) Race ------              6     Northern Leaf Blight (Exserohilum turcicum) Race ------                       Southern Leaf Blight (Bipolaris maydis) Race ------                           Southern Rust (Puccinia polysora)                                       4     Stewart's Witt (Erwinia stewartii)                                            Other (Specify) ------                                                  B. Systemic Diseases                                                          2     Corn Lethal Necrosis (MCMV and MDMV)                                          Head Smut (Sphacelotheca reiliana)                                            Maize Chlorotic Dwarf virus (MDV)                                             Maize Chlorotic Mottle virus (MCMV)                                           Maize Dwarf Mosaic virus (MDMV)                                               Sorghum Downy Mildew of Corn (Peronosclerospora sorghi)                       Other (Specify) ------                                                  C. Stalk Rots                                                                 Anthracnose Stalk Rot (Colletotrichum graminicola)                            Diplodia Stalk Rot (Stenocarpella maydis)                                     Fusarium Stalk Rot (Fusarium moniliforme)                                     Gibberella Stalk Rot (Gibberelia zeae)                                        Other (Specify) ------                                                        D. Ear and Kernel Rots                                                              Aspergillus Ear and Kernel Rot (Aspergillus flavus)                           Diplodia Ear Rot (Stenocarpelia maydis)                                 4     Fusarium Ear and Kernel Rot (Fusarium moniliforme)                      8     Gibberelia Ear Rot (Gibberella zeae)                                          Other (Specify) ------                                                        Banks grass Mite (Oligonychus pratensis)                                      Corn Worm (Helicoverpa zea)                                                    Leaf Feeding                                                                  Silk Feeding                                                                   mg larval wt.                                                               Ear Damage                                                                    Corn Leaf Aphid (Rhopalosiphum maidis)                                        Corn Sap Beetle (Carpophilus dimidiatus                                        European Corn Borer (Ostrinia nubilalis)                               6      1st Genernation (Typically Whorf Leaf Feeding)                         7      2nd Genernation (TypicalIy Leaf Sheath-Collar Feeding)                        Stalk Tunneling                                                              cm tunneled/plant                                                             Fall Armyworm (Spodoptera fruqiperda)                                          Leaf Feeding                                                                  Silk Feeding                                                                  mg larval wt.                                                                Maize Weevil (Sitophilus zea maize                                            Northern Rootworm (Diabrotica barberi)                                        Southern Rootworm (Diabrotica undecimpunctata)                                Southwestem Corn Borer (Diatreaea grandiosella)                                Leaf Feeding                                                                  Stalk Tunneling                                                               cm tunneled/plant                                                            Two-spotted Spider Mite (Tetranychus urticae)                                 Western Rootworm (Diabrotica virgifrea virgifera)                             Other (Specify) ------                                                    AGRONOMIC TRAITS:                                                           5     Stay green (at 65 days after anthesis) (Rate on a scale from 1 =              worst to                                                                      excellent)                                                              0.0   % Dropped Ears (at 65 days affer anthesis)                                    % Pre-anthesis Brittle Snapping                                               % Pre-anthesis Root Lodging                                             2.5   Post-anthesis Root Lodging (at 65 days after anthesis)                  4,970 Kg/ha Yield (at 12-13% grain moisture)                                  __________________________________________________________________________     *In interpreting the foregoing color designations, reference may be had t     the Munsell Glossy Book of Color, a standard color reference.            

Industrial Applicability

This invention also is directed to methods for producing a maize plantby crossing a first parent maize plant with a second parent maize plantwherein either the first or second parent maize plant is an inbred maizeplant of the line PH05G. Further, both first and second parent maizeplants can come from the inbred maize line PH05G. Thus, any such methodsusing the inbred maize line PH05G are part of this invention: selfing,backcrosses, hybrid production, crosses to populations, and the like.All plants produced using inbred maize line PH05G as a parent are withinthe scope of this invention. Advantageously, the inbred maize line isused in crosses with other, different, maize inbreds to produce firstgeneration (F₁) maize hybrid seeds and plants with superiorcharacteristics.

As used herein, the term plant includes plant cells, plant protoplasts,plant cell tissue cultures from which maize plants can be regenerated,plant calli, plant clumps, and plant cells that are intact in plants orparts of plants, such as embryos, pollen, ovules, flowers, kernels,ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk andthe like.

Duncan, Williams, Zehr, and Widholm, Planta (1985) 165:322-332 reflectsthat 97% of the plants cultured that produced callus were capable ofplant regeneration. Subsequent experiments with both inbreds and hybridsproduced 91% regenerable callus that produced plants. In a further studyin 1988, Songstad, Duncan & Widholm in Plant Cell Reports (1988),7:262-265 reports several media additions that enhance regenerability ofcallus of two inbred lines. Other published reports also indicated that"nontraditional" tissues are capable of producing somatic embryogenesisand plant regeneration. K. P. Rao, et al., Maize Genetics CooperationNewsletter, 60:64-65 (1986), refers to somatic embryogenesis from glumecallus cultures and B. V. Conger, et al., Plant Cell Reports, 6:345-347(1987) indicates somatic embryogenesis from the tissue cultures of maizeleaf segments. Thus, it is clear from the literature that the state ofthe art is such that these methods of obtaining plants are, and were,"conventional" in the sense that they are routinely used and have a veryhigh rate of success.

Tissue culture of maize is described in European Patent Application,publication 160,390, incorporated herein by reference. Maize tissueculture procedures are also described in Green and Rhodes, "PlantRegeneration in Tissue Culture of Maize," Maize for Biological Research(Plant Molecular Biology Association, Charlottesville, Va. 1982, at367-372) and in Duncan, et al., "The Production of Callus Capable ofPlant Regeneration from Immature Embryos of Numerous Zea MaysGenotypes," 165 Planta 322-332 (1985). Thus, another aspect of thisinvention is to provide cells which upon growth and differentiationproduce maize plants having the physiological and morphologicalcharacteristics of inbred line PH05G.

Maize is used as human food, livestock feed, and as raw material inindustry. The food uses of maize, in addition to human consumption ofmaize kernels, include both products of dry- and wet-milling industries.The principal products of maize dry milling are grits, meal and flour.The maize wet-milling industry can provide maize starch, maize syrups,and dextrose for food use. Maize oil is recovered from maize germ, whichis a by-product of both dry- and wet-milling industries.

Maize, including both grain and non-grain portions of the plant, is alsoused extensively as livestock feed, primarily for beef cattle, dairycattle, hogs, and poultry.

Industrial uses of maize include production of ethanol, maize starch inthe wetmilling industry and maize flour in the dry-milling industry. Theindustrial applications of maize starch and flour are based onfunctional properties, such as viscosity, film formation, adhesiveproperties, and ability to suspend particles. The maize starch and flourhave application in the paper and textile industries. Other industrialuses include applications in adhesives, building materials, foundrybinders, laundry starches, explosives, oil-well muds, and other miningapplications.

Plant parts other than the grain of maize are also used in industry: forexample, stalks and husks are made into paper and wallboard and cobs areused for fuel and to make charcoal.

The seed of inbred maize line PH05G, the plant produced from the inbredseed, the hybrid maize plant produced from the crossing of the inbred,hybrid seed, and various parts of the hybrid maize plant can be utilizedfor human food, livestock feed, and as a raw material in industry.

PERFORMANCE EXAMPLES OF PH05G

In the examples that follow, the traits and characteristics of inbredmaize line PH05G are given as a line. The data collected on inbred maizeline PH05G is presented for the key characteristics and traits.

Inbred Comparisons

The results in Table 2A compare inbred PH05G to inbred PHTE4. The inbredper se results show inbred PH05G has significantly greater plant heightand significantly higher ear placement than inbred PHTE4. Inbred PH05Gshows significantly higher harvest moisture and significantly lower testweight than inbred PHTE4. Inbred PH05G demonstrates better resistance toroot lodging than inbred PHTE4. Both inbred PH05G and inbred PHTE4demonstrate very good resistance to Goss' Wilt and are highly resistantto Head Smut. Inbred PH05G also shows better resistance to both firstand second brood European Corn Borer than inbred PHTE4

The results in Table 2B compare inbred PH05G to inbred PH38D. The inbredper se results show inbred PH05G demonstrates significantly higher earplacement than inbred PH38D. Inbred PH05G shows significantly higherharvest moisture and significantly lower test weight than inbred PH38D.Inbred PH05G demonstrates good resistance to stalk lodging and exhibitsbetter resistance to root lodging than inbred PH38D. Both inbred PH05Gand inbred PH38D demonstrate very good resistance to Goss' Wilt and bothare highly resistant to Head Smut. Inbred PH05G also shows betterresistance to first brood European Corn Borer and significantly betterresistance to second brood European Corn Borer than inbred PH38D.

The results in Table 2C compare inbred PH05G to inbred PH42B. The inbredper se results show inbred PH05G has higher ear placement than inbredPH42B. Inbred PH05G shows significantly higher harvest moisture andsignificantly lower rest weight than inbred PH42B. Inbred PH05Gdemonstrates better resistance to root lodging than inbred PH42B andboth have high resistance to stalk lodging. Both inbred PH05G and inbredPH42B are highly resistant to Head Smut. Inbred PH05G also shows betterresistance to both first and second brood European Corn Borer thaninbred PH42B.

Inbred By Tester Comparisons

The results in Table 3A compare inbred PH05G to inbred PHTE4, with eachinbred crossed to the same tester lines. The PH05G hybrids produce highyields with significantly lower harvest moisture levels than PHTE4hybrids. The PH05G hybrids exhibit significantly greater plant heightand significantly higher ear placement than the PHTE4 hybrids. Both thePH05G hybrids and the PHTE4 hybrids demonstrate high resistance to rootlodging, stalk lodging and brittle stalk. Both the PH05G hybrids and thePHTE4 hybrids have better than average stay green ratings.

The results in Table 3B compare inbred PH05G to inbred PH38D, with eachinbred crossed to the same tester lines. Both the PH05G hybrids and thePH38D hybrids produce high yields and both hybrids demonstrate lowerthan average harvest moisture levels. The PH05G hybrids exhibitsignificantly greater plant height and significantly higher earplacement than the PH38D hybrids. Both the PH05G hybrids and the PH38Dhybrids are highly resistant to root lodging and stalk lodging. ThePH05G hybrids score significantly higher stay green ratings whencompared to PH38D hybrids.

The results in Table 3C compare inbred PH05G to inbred PH42B, with eachinbred crossed to the same tester lines. Both the PH05G hybrids and thePH42B hybrids produce high yields, with the PH05G hybrids having lowerharvest moisture levels than the PH42B hybrids. The PH05G hybridsexhibit greater plant height and higher ear placement than the PH42Bhybrids. Both the PH05G hybrids and the PH42B hybrids are highlyresistant to root lodging and brittle stalk.

Hybrid Comparisons

The results in Table 4A compare inbred PH05G crossed to inbred PHAP9 andinbred PHP55 crossed to PHTE4. The PH05G/PHAP9 hybrid produces higheryields and has significantly lower harvest moisture levels than thePHP55/ PHTE4 hybrid. The PH05G/PHAP9 hybrid demonstrates significantlygreater plant height and significantly higher ear placement than thePHP55/PHTE4 hybrid. Both hybrids exhibit better than average resistanceto root lodging and the PH05G/PHAP9 hybrid demonstrates significantlybetter resistance to stalk lodging than the PHP55/PHTE4 hybrid. ThePH05G/PHAP9 hybrid scores significantly higher stay green ratings thanthe PHP55/PHTE4 hybrid.

The results in Table 4B compare inbred PH05G crossed to inbred PHAP9 andinbred PHTE4 crossed to PHKW3. The PH05G/PHAP9 hybrid produces aboveaverage yields and has significantly lower harvest moisture levels thanthe PHTE4/PHKW3 hybrid. The PH05G/PHAP9 hybrid demonstratessignificantly greater plant height and significantly higher earplacement than the PHTE4/PHKW3 hybrid. The PH05G/PHAP9 hybrid exhibitssignificantly better resistance to stalk lodging than the PHTE4/PHKW3hybrid. The PH05G/PHAP9 hybrid scores a significantly higher stay greenrating than the PHTE4/PHAP9 hybrid.

The results in Table 4C compare inbred PH05G crossed to inbred PHNG2 andinbred PH42B crossed to PH06N. The PH05G/PHNG2 hybrid produces aboveaverage yields and has significantly lower harvest moisture levels thanthe PH42B/PH06N hybrid. The PH05G/PHNG2 hybrid demonstratessignificantly greater plant height and significantly higher earplacement than the PH42B/PH06N hybrid. The PH05G/PHNG2 hybrid scores ahigher stay green rating than the PH42B/PH06N hybrid. The PH05G/PHNG2hybrid exhibits significantly better resistance to Gray Leaf Spot thanthe PH42B/PH06N hybrid.

The results in Table 4D compare inbred PH05G crossed to inbred PHNG2 andinbred PHJ90 crossed to PHTE4. The PH05G/PHNG2 hybrid producessignificantly higher yields than the PHJ90/PHTE4 hybrid. The PH05G/PHNG2hybrid demonstrates significantly greater plant height and significantlyhigher ear placement than the PHJ90/PHTE4 hybrid. The PH05G/PHNG2 hybridexhibits above average resistance to stalk lodging. The PH05G/PHNG2hybrid scores a significantly higher stay green rating than thePHJ90/PHTE4 hybrid.

The results in Table 5A compare inbred PH05G crossed to inbred PHAP9 andinbred PHRF5 crossed to PHJ89. The PH05G/PHAP9 hybrid producessignificantly more tonnage of silage than the PHRF5/PHJ89 hybrid.

The results in Table 5B compare inbred PH05G crossed to inbred PHAP9 andinbred PHP55 crossed to PHTE4. The PH05G/PHAP9 hybrid producessignificantly more tonnage of silage than the PHP55/PHTE4 hybrid.

The results in Table 5C compare inbred PH05G crossed to inbred PHNG2 andinbred PHR61 crossed to PHK56. The PH05G/PHNG2 hybrid produces moretonnage of silage than the PHR61/PHK56 hybrid.

                                      TABLE 2A                                    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT                                               VARIETY #1 = PH05G                                                            VARIETY #2 = PHTE4                                                            __________________________________________________________________________               BU  BU      TST SDG EST  TIL GDU  GDU                                         ACR ACR MST WT  VGR CNT  LER SHD  SLK                                         ABS % MN                                                                              ABS ABS ABS ABS  ABS ABS  ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   68.0                                                                              98  23.9                                                                              53.5                                                                              4.8 32.7 3.7 136.0                                                                              140.9                                   2   69.3                                                                              99  22.1                                                                              56.5                                                                              5.1 35.3 3.3 131.5                                                                              131.7                                   LOCS                                                                              42  42  50  37  66  102  65  96   90                                      REPS                                                                              62  62  71  54  74  144  78  104  96                                      DIFF                                                                              1.3 2   1.8 2.9 0.3 2.6  0.4 4.5  9.2                                     PR > T                                                                            .687                                                                              .749                                                                              .004#                                                                             .000#                                                                             .134                                                                              .000#                                                                              .712                                                                              .000#                                                                              .000#                            __________________________________________________________________________               POL TAS PLT  EAR RT  STA  STK BRT GRN                                         SC  SZ  HT   HT  LDG GRN  LDG STK APP                                         ABS ABS ABS  ABS ABS ABS  ABS ABS ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   4.5 4.4 83.6 32.7                                                                              98.4                                                                              5.1  96.1                                                                              93.5                                                                              6.5                                     2   4.5 4.6 77.1 28.2                                                                              93.3                                                                              4.9  93.3                                                                              92.4                                                                              6.9                                     LOCS                                                                              13  43  53   43  19  20   25  12  16                                      REPS                                                                              14  45  62   51  23  23   38  13  23                                      DIFF                                                                              0.1 0.2 6.5  4.4 5.1 0.3  2.9 1.1 0.4                                     PR > T                                                                            .874                                                                              .347                                                                              .000#                                                                              .000#                                                                             .103                                                                              .556 .292                                                                              .760                                                                              .337                             __________________________________________________________________________               SCT  EAR TEX EAR BAR  DRP GLF  NLF GOS                                        GRN  SZ  EAR MLD FLT  EAR SPT  BLT WLT                                        ABS  ABS ABS ABS ABS  ABS ABS  ABS ABS                             __________________________________________________________________________    TOTAL SUM                                                                            1   6.5  5.9 5.3 6.5 93.6 99.4                                                                              3.5  6.1 7.0                                    2   7.4  4.4 5.8 7.4 96.8 99.1                                                                              3.2  5.8 8.0                                    LOCS                                                                              14   8   8   12  50   19  9    8   2                                      REPS                                                                              15   8   9   13  53   28  14   13  4                                      DIFF                                                                              0.9  1.5 0.4 0.9 3.2  0.3 0.3  0.3 1.0                                    PR > T                                                                            .084*                                                                              .020+                                                                             .371                                                                              .042+                                                                             .010+                                                                              .092*                                                                             .302 .329                                                                              .295                            __________________________________________________________________________               STW HD       FUS                                                                              GIB   EYE                                                                              COM   HC ECB                                         WLT SMT CLN  ERS                                                                              ERS   SPT                                                                              RST   BLT                                                                              1LF                                         ABS ABS ABS  ABS                                                                              ABS   ABS                                                                              ABS   ABS                                                                              ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   3.5 99.1                                                                              2.0  4.0                                                                              6.2   7.5                                                                              4.5   6.0                                                                              6.0                                     2   4.3 100.0                                                                             4.0  3.0                                                                              4.5   4.5                                                                              5.8   4.0                                                                              5.6                                     LOCS                                                                              2   3   1    1  3     2  3     1  8                                       REPS                                                                              3   8   2    1  6     2  5     1  8                                       DIFF                                                                              0.8 0.9 2.0  1.0                                                                              1.7   3.0                                                                              1.3   2.0                                                                              0.4                                     PR > T                                                                            .742                                                                              .423        .010+ .374                                                                             .057*    .608                             __________________________________________________________________________               ECB                                                                           2SC                                                                           ABS                                                                __________________________________________________________________________    TOTAL SUM                                                                            1   7.3                                                                       2   4.5                                                                       LOCS                                                                              3                                                                         REPS                                                                              4                                                                         DIFF                                                                              2.8                                                                       PR > T                                                                            .023+                                                              __________________________________________________________________________     *10% SIG                                                                      +5% SIG                                                                       #1% SIG                                                                  

                                      TABLE 2B                                    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT                                               VARIETY #1 = PH05G                                                            VARIETY #2 = PH38D                                                            __________________________________________________________________________               BU  BU      TST SDG EST  TIL GDU  GDU                                         ACR ACR MST WT  VGR CNT  LER SHD  SLK                                         ABS % MN                                                                              ABS ABS ABS ABS  ABS ABS  ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   64.4                                                                              94  25.5                                                                              53.4                                                                              4.8 37.5 3.3 136.0                                                                              141.0                                   2   66.0                                                                              96  22.2                                                                              56.0                                                                              5.0 40.4 1.3 140.1                                                                              141.4                                   LOCS                                                                              66  66  72  55  70  122  73  95   88                                      REPS                                                                              91  91  98  75  82  168  88  102  93                                      DIFF                                                                              1.7 2   3.3 2.6 0.2 2.9  2.0 4.1  0.4                                     PR > T                                                                            .497                                                                              .597                                                                              .000#                                                                             .000#                                                                             .411                                                                              .000#                                                                              .119                                                                              .000#                                                                              .488                             __________________________________________________________________________               POL TAS TAS PLT EAR  RT  STA STK  BRT                                         SC  BLS SZ  HT  HT   LDG GRN LDG  STK                                         ABS ABS ABS ABS ABS  ABS ABS ABS  ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   4.5 9.0 4.3 83.7                                                                              32.8                                                                              96.7 5.3 96.6 94.2                                    2   4.9 9.0 3.3 82.6                                                                              29.3                                                                              91.4 4.4 94.5 94.8                                    LOCS                                                                              14  1   40  53  44  26   19  36   15                                      REPS                                                                              15  1   42  62  53  31   22  52   16                                      DIFF                                                                              0.4 0.0 1.0 1.2 3.5 5.3  0.9 2.1  0.6                                     PR > T                                                                            .327    .001#                                                                             .180                                                                              .000#                                                                             .063*                                                                              .161                                                                              .267 .704                             __________________________________________________________________________               GRN SCT EAR TEX EAR BAR  DRP GLF NLF                                          APP GRN SZ  EAR MLD PLT  EAR SPT BLT                                          ABS ABS ABS ABS ABS ABS  ABS ABS ABS                               __________________________________________________________________________    TOTAL SUM                                                                            1   6.2 6.2 5.8 5.3 6.3 93.8 99.5                                                                              3.8 6.1                                      2   7.0 7.0 5.0 6.3 6.9 95.7 99.0                                                                              2.7 5.9                                      LOCS                                                                              19  16  9   9   15  51   27  9   8                                        REPS                                                                              26  17  9   10  16  54   39  13  13                                       DIFF                                                                              0.8 0.8 0.8 1.1 0.7 1.9  0.5 1.1 0.2                                      PR > T                                                                            .001#                                                                             .160                                                                              .133                                                                              .023+                                                                             .126                                                                              .270 .041+                                                                             .008#                                                                             .714                              __________________________________________________________________________               GOS                                                                              STW  HD       GIB EYE  COM HC  ECB                                         WLT                                                                              WLT  SMT CLN  ERS SPT  RST BLT 1LF                                         ABS                                                                              ABS  ABS ABS  ABS ABS  ABS ABS ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   7.0                                                                              3.5  98.6                                                                              2.0  6.0 7.0  5.4 6.0 6.0                                     2   8.0                                                                              3.3  100.0                                                                             6.5  4.3 8.0  4.4 4.0 4.9                                     LOCS                                                                              2  2    2   1    2   1    4   1   8                                       REPS                                                                              4  3    6   2    4   1    6   1   8                                       DIFF                                                                              1.0                                                                              0.3  1.4 4.5  1.8 1.0  1.0 2.0 1.1                                     PR > T                                                                            .626                                                                             .795 .500     .090*    .066*   .038+                            __________________________________________________________________________               ECB                                                                           2SC                                                                           ABS                                                                __________________________________________________________________________    TOTAL SUM                                                                            1   6.9                                                                       2   4.1                                                                       LOCS                                                                              5                                                                         REPS                                                                              8                                                                         DIFF                                                                              2.8                                                                       PR > T                                                                            .006#                                                              __________________________________________________________________________     *10% SIG                                                                      +5% SIG                                                                       #1% SIG                                                                  

                                      TABLE 2C                                    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT                                               VARIETY #1 = PH05G                                                            VARIETY #2 = PH42B                                                            __________________________________________________________________________               BU  BU      TST SDG EST  TIL GDU  GDU                                         ACR ACR MST WT  VGR CNT  LER SHD  SLK                                         ABS % MN                                                                              ABS ABS ABS ABS  ABS ABS  ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   69.2                                                                              99  24.7                                                                              53.8                                                                              4.8 35.1 3.7 136.1                                                                              141.1                                   2   69.1                                                                              100 22.9                                                                              55.0                                                                              5.0 38.0 2.7 142.1                                                                              145.2                                   LOCS                                                                              49  49  52  42  59  100  66  88   83                                      REPS                                                                              72  72  76  61  70  142  80  96   89                                      DIFF                                                                              0.1 1   1.8 1.2 0.2 2.9  1.0 6.0  4.1                                     PR > T                                                                            .976                                                                              .871                                                                              .003#                                                                             .000#                                                                             .298                                                                              .000#                                                                              .608                                                                              .000#                                                                              .000#                            __________________________________________________________________________               POL TAS PLT  EAR RT  STA  STK BRT GRN                                         SC  SZ  HT   HT  LDG GRN  LDG STK APP                                         ABS ABS ABS  ABS ABS ABS  ABS ABS ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   4.7 4.4 83.2 32.9                                                                              97.5                                                                              5.1  96.4                                                                              94.5                                                                              6.5                                     2   3.0 1.9 83.7 30.9                                                                              91.7                                                                              6.9  96.8                                                                              92.8                                                                              6.1                                     LOCS                                                                              11  38  47   37  19  18   30  11  15                                      REPS                                                                              12  40  55   45  24  21   44  12  21                                      DIFF                                                                              1.8 2.5 0.5  2.0 5.7 1.7  0.5 1.7 0.4                                     PR > T                                                                            .002#                                                                             .000#                                                                             .649 .014+                                                                             .014+                                                                             .013+                                                                              .789                                                                              .570                                                                              .279                             __________________________________________________________________________               SCT  EAR TEX EAR BAR DRP   GLF NLF                                                                              STW                                         GRN  SZ  EAR MLD PLT EAR   SFT BLT                                                                              WLT                                         ABS  ABS ABS ABS ABS ABS   ABS ABS                                                                              ABS                              __________________________________________________________________________    TOTAL SUM                                                                            1   6.1  5.6 5.0 6.0 93.9                                                                              99.5  3.3 8.0                                                                              3.0                                     2   5.6  7.0 5.6 6.8 93.9                                                                              99.8  5.3 8.5                                                                              7.0                                     LOCS                                                                              14   8   8   12  39  22    6   2  1                                       REPS                                                                              15   8   8   13  42  32    6   2  1                                       DIFF                                                                              0.5  1.4 0.6 0.8 0.1 0.3   2.0 0.5                                                                              4.0                                     PR > T                                                                            .314 .008#                                                                             .180                                                                              .145                                                                              .970                                                                              .377  .013+                                                                             .500                                __________________________________________________________________________               HD     FUS                                                                              EYE    COM HC     ECB ECB                                           SMT    ERS                                                                              SPT    RST BLT    1LF 2SC                                           ABS    ABS                                                                              ABS    ABS ABS    ABS ABS                                __________________________________________________________________________    TOTAL SUM                                                                            1   98.2   4.0                                                                              7.5    4.5 6.0    6.1 6.9                                       2   98.9   3.0                                                                              6.5    3.8 5.5    4.5 4.7                                       LOCS                                                                              1      1  2      3   1      7   5                                         REPS                                                                              4      1  2      5   1      7   8                                         DIFF                                                                              0.6    1.0                                                                              1.0    0.7 0.5    1.7 2.2                                       PR > T        .500   .057*      .015+                                                                             .017+                              __________________________________________________________________________     *10% SIG                                                                      +5% SIG                                                                       #1% SIG                                                                  

                                      TABLE 3A                                    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH05G To PHTE4 Crossed         To The Same Inbred Testers And Grown In The Same Experiments.                 __________________________________________________________________________               SEL     BU  BU  PRM     TST                                                                              SDG EST GDU                                        IND PRM ACR ACR SHD MST WT VGR CNT SHD                                        % MN                                                                              ABS ABS % MN                                                                              ABS % MN                                                                              ABS                                                                              % MN                                                                              % MN                                                                              % MN                            __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              4   7   45  45  7   45  34 18  18  16                                     LOCS                                                                              4   7   43  43  7   43  32 17  16  15                                     PH05G                                                                             107 101 146 99  103 98  54 105 103 101                                    PHTE4                                                                             94  104 146 100 100 102 56 108 101 99                                     DIFF                                                                              13  3   0   1   3   5   2  3   2   2                                      PR > T                                                                            0.11                                                                              0.00                                                                              0.99                                                                              0.81                                                                              0.07                                                                              0.00                                                                              0.00                                                                             0.70                                                                              0.46                                                                              0.01                            __________________________________________________________________________               GDU STK PLT EAR RT  STA STK BRT GRN EYE                                       SLK CNT HT  HT  LDG GRN LDG STK APP SPT                                       % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              ABS                            __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              6   48  19  19  16  21  40  5   18  1                                     LOCS                                                                              6   45  19  19  15  20  39  5   16  1                                     PH05G                                                                             102 100 104 111 101 106 98  100 96  7                                     PHTE4                                                                             97  102 98  96  105 109 101 100 92  5                                     DIFF                                                                              5   2   6   15  4   4   3   0   4   2                                     PR > T                                                                            0.01                                                                              0.27                                                                              0.00                                                                              0.00                                                                              0.40                                                                              0.70                                                                              0.22                                                                              0.99                                                                              0.37                               __________________________________________________________________________               ECB ECB DRP                                                                   DPE 2SC EAR                                                                   ABS ABS % MN                                                       __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              9   5   36                                                                LOCS                                                                              9   5   35                                                                PH05G                                                                             100 3   99                                                                PHTE4                                                                             100 4   100                                                               DIFF                                                                              0   1   0                                                                 PR > T                                                                            0.99                                                                              0.45                                                                              0.99                                                       __________________________________________________________________________     *PR > T values are valid only for comparison with Locs >= 10.            

                                      TABLE 3B                                    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH05G To PH38D Crossed         To The Same Inbred Testers And Grown In The Same Experiments.                 __________________________________________________________________________               SEL     BU  BU  PRM     TST SDG EST GDU                                       IND PRM ACR ACR SHD MST WT  VGR CNT SHD                                       % MN                                                                              ABS ABS % MN                                                                              ABS % MN                                                                              ABS % MN                                                                              % MN                                                                              % MN                           __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              27  41  209 209 37  209 141 98  87  68                                    LOCS                                                                              27  41  204 204 37  204 136 93  82  66                                    PH05G                                                                             98  100 139 98  103 96  54  95  99  100                                   PH38D                                                                             98  101 138 98  105 96  56  96  100 101                                   DIFF                                                                              0   0   1   0   1   0   1   1   1   1                                     PR > T                                                                            0.99                                                                              0.99                                                                              0.73                                                                              0.99                                                                              0.00                                                                              0.99                                                                              0.00                                                                              0.68                                                                              0.22                                                                              0.00                           __________________________________________________________________________               GDU STK PLT EAR RT  STA STK BRT GRN ECB                                       SLK CNT HT  HT  LDG GRN LDG STK APP DPE                                       % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              ABS                            __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              35  225 91  91  92  96  193 31  50  19                                    LOCS                                                                              34  217 90  90  90  92  189 29  47  19                                    PH05G                                                                             101 99  103 107 102 100 100 89  91  100                                   PH38D                                                                             101 99  99  97  99  71  101 98  102 98                                    DIFF                                                                              0   0   4   10  3   28  1   8   11  2                                     PR > T                                                                            0.99                                                                              0.99                                                                              0.00                                                                              0.00                                                                              0.17                                                                              0.00                                                                              0.18                                                                              0.02                                                                              0.00                                                                              0.05                           __________________________________________________________________________               ECB                                                                              ECB                                                                              DRP NLF                                                                 1LF                                                                              2SC                                                                              EAR BLT                                                                 ABS                                                                              ABS                                                                              % MN                                                                              ABS                                                      __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              4  36 155 1                                                               LOCS                                                                              4  36 152 1                                                               PH05G                                                                             5  4  100 8                                                               PH38D                                                                             3  3  99  6                                                               DIFF                                                                              2  1  0   2                                                               PR > T                                                                            0.07                                                                             0.00                                                                             0.99                                                         __________________________________________________________________________     *PR > T values are valid only for comparisons with Locs >= 10.           

                                      TABLE 3C                                    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH05G To PH42B Crossed         To The Same Inbred Testers And Grown In The Same Experiments.                 __________________________________________________________________________               SEL     BU  BU  PRM     TST SDG EST GDU                                       IND PRM ACR ACR SHD MST WT  VGR CNT SHD                                       % MN                                                                              ABS ABS % MN                                                                              ABS % MN                                                                              ABS % MN                                                                              % MN                                                                              % MN                           __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              3   5   30  30  5   30  24  23  15  9                                     LOCS                                                                              3   5   28  28  5   28  22  21  13  9                                     PH05G                                                                             98  103 153 100 101 97  54  91  99  98                                    PH42B                                                                             109 104 159 103 107 100 55  96  99  102                                   DIFF                                                                              11  2   6   3   6   3   1   4   0   4                                     PR > T                                                                            0.25                                                                              0.08                                                                              0.13                                                                              0.20                                                                              0.02                                                                              0.02                                                                              0.00                                                                              0.35                                                                              0.99                                                                              0.00                           __________________________________________________________________________               GDU STK PLT EAR RT  STA STK BRT GRN ECB                                       SLK CNT HT  HT  LDG GRN LDG STK APP DPE                                       % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              % MN                                                                              ABS                            __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              4   36  12  12  8   18  30  3   19  4                                     LOCS                                                                              4   32  12  12  8   16  28  3   17  4                                     PH05G                                                                             99  99  103 107 100 80  98  102 94  100                                   PH42B                                                                             102 99  100 97  102 115 106 102 99  100                                   DIFF                                                                              3   0   4   10  1   36  7   0   5   1                                     PR > T                                                                            0.09                                                                              0.99                                                                              0.04                                                                              0.02                                                                              0.26                                                                              0.00                                                                              0.00                                                                              0.99                                                                              0.22                                                                              0.39                           __________________________________________________________________________               ECB                                                                              ECB                                                                              DRP                                                                     1LF                                                                              2SC                                                                              EAR                                                                     ABS                                                                              ABS                                                                              % MN                                                         __________________________________________________________________________    TOTAL SUM                                                                            REPS                                                                              1  6  28                                                                  LOCS                                                                              1  6  26                                                                  PH05G                                                                             4  4  100                                                                 PH42B                                                                             3  4  100                                                                 DIFF                                                                              1  0  0                                                                   PR > T 0.99                                                                             0.99                                                         __________________________________________________________________________     *PR > T values are valid only for comparisons with Locs >= 10.           

                                      TABLE 4A                                    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT                                          VARIETY #1 = PH05G/PHAP9                                                      VARIETY #2 = PHP55/PHTE4                                                      __________________________________________________________________________                    PRM BU   BU        TST SDG  EST  GDU                                     PRM  SHD ACR  ACR  MST  WT  VGR  CNT  SHD                                     ABS  ABS ABS  % MN % MN ABS % MN % MN % MN                         __________________________________________________________________________    TOTAL SUM                                                                            1   100  101 153.7                                                                              105  98   54.0                                                                              90   98   99                                  2   103  102 149.4                                                                              102  102  54.3                                                                              98   104  100                                 LOCS                                                                              23   38  96   96   98   67  88   114  68                                  REPS                                                                              23   38  114  114  116  81  109  132  78                                  DIFF                                                                              2    0   4.3  3    4    0.3 9    6    0                                   PR > T                                                                            .000#                                                                              .999                                                                              .048+                                                                              .039+                                                                              .000#                                                                              .069*                                                                             .001#                                                                              .000#                                                                              .999                         __________________________________________________________________________               GDU  STK  PLT  EAR  RT  STA  STK  BRT GRN                                     SLK  CNT  HT   HT   LDG GRN  LDG  STK APP                                     % MN % MN % MN % MN % MN                                                                              % MN % MN % MN                                                                              % MN                         __________________________________________________________________________    TOTAL SUM                                                                            1   101  99   105  109  101 118  102  100 97                                  2   100  102  100  100  101 89   99   99  84                                  LOCS                                                                              41   215  89   89   50  71   87   20  19                                  REPS                                                                              49   284  111  111  65  87   103  31  24                                  DIFF                                                                              1    3    5    9    1   29   4    1   13                                  PR > T                                                                            .001#                                                                              .000#                                                                              .000#                                                                              .000#                                                                              .854                                                                              .000#                                                                              .009#                                                                              .710                                                                              .020+                        __________________________________________________________________________               DRP   GLF NLF   GOS ANT  HD  GIB  EYE                                                                              ECB                                      EAR   SPT BLT   WLT ROT  SMT ERS  SPT                                                                              DPE                                      % MN  ABS ABS   ABS ABS  ABS ABS  ABS                                                                              ABS                           __________________________________________________________________________    TOTAL SUM                                                                            1   100   3.8 5.8   7.5 2.5  100.0                                                                             5.3  6.3                                                                              98.3                                 2   100   3.3 4.3   7.5 3.5  86.9                                                                              2.8  4.5                                                                              99.5                                 LOCS                                                                              65    6   8     3   1    2   2    4  15                                   REPS                                                                              77    12  16    6   2    6   3    4  16                                   DIFF                                                                              0     0.5 1.4   0.0 1.0  13.1                                                                              2.5  1.8                                                                              1.1                                  PR > T                                                                            .999  .296                                                                              .001# .999     .472                                                                              .126 .188                                                                             .021+                         __________________________________________________________________________               ECB ECB                                                                       1LF 2SC                                                                       ABS ABS                                                            __________________________________________________________________________    TOTAL SUM                                                                            1   6.0 4.4                                                                   2   5.5 3.2                                                                   LOCS                                                                              2   9                                                                     REPS                                                                              2   10                                                                    DIFF                                                                              0.5 1.2                                                                   PR > T                                                                            .500                                                                              .017+                                                          __________________________________________________________________________     *10% SIG                                                                      +5% SIG                                                                       #1% SIG                                                                  

                                      TABLE 4B                                    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT                                          VARIETY #1 = PH05G/PHAP9                                                      VARIETY #2 = PHTE4/PHKW3                                                      __________________________________________________________________________                    PRM  BU  BU       TST  SDG  EST  GDU                                     PRM  SHD  ACR ACR  MST WT   VGR  CNT  SHD                                     ABS  ABS  ABS % MN % MN                                                                              ABS  % MN % MN % MN                         __________________________________________________________________________    TOTAL SUM                                                                            1   100  101  143.1                                                                             103  94  54.8 87   97   98                                  2   102  101  140.5                                                                             100  99  56.8 107  100  98                                  LOCS                                                                              13   24   54  54   55  33   60   57   42                                  REPS                                                                              13   24   59  59   60  37   71   66   45                                  DIFF                                                                              2    0    2.7 3    5   2.1  20   3    0                                   PR > T                                                                            .002#                                                                              .999 .352                                                                              .254 .000#                                                                             .000#                                                                              .000#                                                                              .142 .999                         __________________________________________________________________________               GDU  STK  PLT  EAR  RT  STA  STK  BRT GRN                                     SLK  CNT  HT   HT   LDG GRN  LDG  STK APP                                     % MN % MN % MN % MN % MN                                                                              % MN % MN % MN                                                                              % MN                         __________________________________________________________________________    TOTAL SUM                                                                            1   100  99   104  106  97  118  103  98  103                                 2   98   101  99   98   92  82   96   110 97                                  LOCS                                                                              28   133  48   48   30  44   52   15  3                                   REPS                                                                              30   177  62   62   36  54   59   24  3                                   DIFF                                                                              2    3    5    8    5   36   7    12  6                                   PR > T                                                                            .001#                                                                              .032+                                                                              .000#                                                                              .000#                                                                              .326                                                                              .000#                                                                              .002#                                                                              .176                                                                              .764                         __________________________________________________________________________               DRP  GLF   NLF GOS ANT  HD  GIB  ECB ECB                                      EAR  SPT   BLT WLT ROT  SMT ERS  DPE 2SC                                      % MN ABS   ABS ABS ABS  ABS ABS  ABS ABS                           __________________________________________________________________________    TOTAL SUM                                                                            1   100  3.8   5.5 7.0 2.5  100.0                                                                             5.3  98.2                                                                              4.3                                  2   100  3.6   4.2 6.7 3.0  97.2                                                                              5.0  99.2                                                                              3.6                                  LOCS                                                                              33   5     7   3   1    2   2    11  8                                    REPS                                                                              36   10    14  5   2    6   3    11  9                                    DIFF                                                                              1    0.2   1.3 0.3 0.5  2.8 0.3  1.0 0.8                                  PR > T                                                                            .004#                                                                              .587  .012+                                                                             .742     .126                                                                              .874 .054*                                                                             .402                          __________________________________________________________________________     *10% SIG                                                                      +5% SIG                                                                       #1% SIG                                                                  

                                      TABLE 4C                                    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT                                          VARIETY #1 = PH05G/PHNG2                                                      VARIETY #2 = PH42B/PH06N                                                      __________________________________________________________________________                    PRM BU   BU       TST  SDG EST  GDU                                      PRM  SHD ACR  ACR MST  WT   VGR CNT  SHD                                      ABS  ABS ABS  % MN                                                                              % MN ABS  % MN                                                                              % MN % MN                          __________________________________________________________________________    TOTAL SUM                                                                            1   106  106 154.7                                                                              105 98   55.6 99  96   99                                   2   107  106 156.2                                                                              106 101  55.8 97  91   99                                   LOCS                                                                              17   36  79   79  81   55   100 122  72                                   REPS                                                                              17   36  91   91  93   64   113 136  79                                   DIFF                                                                              1    0   1.5  1   3    0.2  1   5    0                                    PR > T                                                                            .012+                                                                              .999                                                                              .495 .356                                                                              .000#                                                                              .287 .566                                                                              .000#                                                                              .999                          __________________________________________________________________________               GDU STK  PLT  EAR  RT  STA  STK  ERT GRN                                      SLK CNT  HT   HT   LDG GRN  LDG  STK APP                                      % MN                                                                              % MN % MN % MN % MN                                                                              % MN % MN % MN                                                                              % MN                          __________________________________________________________________________    TOTAL SUM                                                                            1   100 98   102  102  96  127  101  88  96                                   2   100 96   100  98   102 116  107  96  98                                   LOCS                                                                              42  246  97   97   41  69   67   17  19                                   REPS                                                                              47  293  106  106  47  78   76   28  21                                   DIFF                                                                              0   2    3    4    6   11   6    8   2                                    PR > T                                                                            .999                                                                              .001#                                                                              .000#                                                                              .000#                                                                              .400                                                                              .020+                                                                              .008#                                                                              .267                                                                              .660                          __________________________________________________________________________               DRP  GLF NLF   GOS                                                                              HD    GIB                                                                              ECB   ECB                                                                              ECB                                       EAR  SPT BLT   WLT                                                                              SMT   ERS                                                                              DPE   1LF                                                                              2SC                                       % MN ABS ABS   ABS                                                                              ABS   ABS                                                                              ABS   ABS                                                                              ABS                            __________________________________________________________________________    TOTAL SUM                                                                            1   100  5.0 4.8   8.0                                                                              100.0 5.0                                                                              99.1  5.0                                                                              4.2                                   2   100  4.0 5.5   7.5                                                                              99.2  7.0                                                                              99.4  3.5                                                                              3.8                                   LOCS                                                                              46   11  4     1  3     1  7     2  9                                     REPS                                                                              54   14  8     2  10    1  8     2  9                                     DIFF                                                                              0    1.0 0.8   0.5                                                                              0.8   2.0                                                                              0.3   1.5                                                                              0.4                                   PR > T                                                                            .999 .007#                                                                             .476     .423     .693  .500                                                                             .225                           __________________________________________________________________________     *10% SIG                                                                      +5% SIG                                                                       #1% SIG                                                                  

                                      TABLE 4D                                    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT                                          VARIETY #1 = PH05G/PHNG2                                                      VARIETY #2 = PHJ90/PHTE4                                                      __________________________________________________________________________                   PPM  BU   BU        TST SDG EST GDU                                       PRM SHD  ACR  ACR  MST  WT  VGR CNT SHD                                       ABS ABS  ABS  % MN % MN ABS % MN                                                                              % MN                                                                              % MN                           __________________________________________________________________________    TOTAL SUM                                                                            1   104 105  161.3                                                                              107  102  54.4                                                                              104 99  101                                   2   103 100  142.7                                                                              95   99   55.3                                                                              96  97  96                                    LOCS                                                                              9   14   39   39   40   22  20  29  26                                    REPS                                                                              9   14   53   53   54   28  30  36  33                                    DIFF                                                                              1   6    18.6 12   3    0.8 8   2   5                                     PR > T                                                                            .014+                                                                             .000#                                                                              .000#                                                                              .000#                                                                              .050*                                                                              .057*                                                                             .138                                                                              .494                                                                              .000#                          __________________________________________________________________________               GDU  STK PLT  EAR  RT  STA  STK BRT  DRP                                      SLK  CNT HT   HT   LDG GRN  LDG STK  EAR                                      % MN % MN                                                                              % MN % MN % MN                                                                              % MN % MN                                                                              % MN % MN                          __________________________________________________________________________    TOTAL SUM                                                                            1   101  100 104  103  100 133  104 84   100                                  2   96   99  94   94   104 69   101 90   100                                  LOCS                                                                              15   58  23   23   23  19   32  4    25                                   REPS                                                                              18   86  31   31   32  27   43  10   31                                   DIFF                                                                              5    1   10   9    5   64   3   7    0                                    PR > T                                                                            .000#                                                                              .327                                                                              .000#                                                                              .001#                                                                              .244                                                                              .000#                                                                              .109                                                                              .562 .999                          __________________________________________________________________________               ECB ECB                                                                              ECB                                                                    DFE 1LF                                                                              2SC                                                                    ABS ABS                                                                              ABS                                                         __________________________________________________________________________    TOTAL SUM                                                                            1   87.3                                                                              6.0                                                                              3.7                                                                2   85.4                                                                              7.0                                                                              2.5                                                                LOCS                                                                              12  1  6                                                                  REPS                                                                              17  1  6                                                                  DIFF                                                                              1.9 1.0                                                                              1.2                                                                PR > T                                                                            .268   .034+                                                       __________________________________________________________________________     *10% SIG                                                                      +5% SIG                                                                       #1% SIG                                                                  

                  TABLE 5A                                                        ______________________________________                                        SILAGE PAIRED COMPARISON REPORT                                               VARIETY #1 = PH05G/PHAP9                                                      VARIETY #2 = PHRF5/PHJ89                                                                         SI/                                                                           WTA                                                                           ABS                                                        ______________________________________                                        TOTAL SUM        1       24.1                                                                  2       22.1                                                                  LOCS    20                                                                    REPS    40                                                                    DIFF    2.0                                                                   PROB     .001#                                               ______________________________________                                         * = 10% SIG                                                                   + = 5% SIG                                                                    # = 1% SIG                                                               

                  TABLE 5B                                                        ______________________________________                                        SILAGE PAIRED COMPARISON REPORT                                               VARIETY #1 = PH05G/PHAP9                                                      VARIETY #2 = PHP55/PHTE4                                                                         SI/                                                                           WTA                                                                           ABS                                                        ______________________________________                                        TOTAL SUM        1       24.6                                                                  2       22.9                                                                  LOCS    18                                                                    REPS    36                                                                    DIFF    1.6                                                                   PROB    .005#                                                ______________________________________                                         *10% SIG                                                                      +5% SIG                                                                       #1% SIG                                                                  

                  TABLE 5C                                                        ______________________________________                                        SILAGE PAIRED COMPARISON REPORT                                               VARIETY #1 = PH05G/PHNG2                                                      VARIETY #2 = PHR61/PHK56                                                                         SI/                                                                           WTA                                                                           ABS                                                        ______________________________________                                        TOTAL SUM        1       24.5                                                                  2       23.7                                                                  LOCS    23                                                                    REPS    45                                                                    DIFF    0.8                                                                   PROB    .118                                                 ______________________________________                                         * = 10% SIG                                                                   + = 5% SIG                                                                    # = 1% SIG                                                               

Applicant has made a deposit of at least 2500 seeds of Inbred Maize LinePHO05G with the American Type Culture Collection (ATCC), Manassas, Va.201110 USA, ATCC Deposit No. 209728. The seeds deposited with the ATCCon Apr. 2, 1998 were taken from the deposit maintained by PioneerHi-Bred International, Inc. 700 Capital Square, 400 Locust Streer, DesMoines, Iowa 503209-2340 since prior to the filing date of thisapplication. This deposit of the Inbred Maize Line PH05G will bemaintained in the ATCC depository, which is a public depository, for aperiod of 30 years, or 5 years after the most recent request, or for theeffective life of the patent, whichever is longer, and will be replacedif it becomes nonviable during that period. Additionally, Applicant hassatisfied all the requirements of 37 C.F.R. §§.801-1.809, includingproviding an indication of the viability of the sample availability ofthe deposited material from the ATCC; however, Applicant has noauthority to waive any restrictions imposed by law on the transfer ofbiological material or its transportation in commerce. Applicant doesnot waive any infringement of its rights granted under this patent orunder the Plant Valley Protection Act (7 USC 2321 et seq.). U.S. PlantVariety Protection of PH05G has been applied for under application Ser.No. 9700211.

The foregoing invention has been described in detail by way ofillustration and example for purposes of clarity and understanding.However, it will be obvious that certain changes and modifications suchas single gene modifications and mutations, somoclonal variants, variantindividuals selected from large populations of the plants of the instantinbred and the like may be practiced within the scope of the invention,as limited only by the scope of the appended claims.

What is claimed is:
 1. Seed of maize inbred line designated PH05G,representative samples having been deposited under ATCC Accession No.209728.
 2. A maize plant, or parts thereof, having all the physiologicaland morphological characteristics of inbred line PH05G, representativeseed of said line having been deposited under ATCC accession No. 209728.3. The maize plant of claim 2, wherein said plant is male sterile.
 4. Atissue culture of regenerable cells of a maize plant of inbred linePH05G, wherein the tissue regenerates plants capable of expressing allthe morphological and physiological characteristics of the inbred linePH05G, representative seed of which have been deposited under ATCCAccession No.
 209728. 5. A tissue culture according to claim 4, thecells or protoplasts being from a tissue selected from the groupconsisting of leaves, pollen, embryos, roots, root tips, anthers, silks,flowers, kernels, ears, cobs, husks, and stalks.
 6. A maize plantregenerated from the tissue culture of claim 4, said plants, capable ofexpressing all the morphological and physiological characteristics ofinbred line PH05G, representative seed of which have been depositedunder ATCC Accession No.
 209728. 7. A method for producing a firstgeneration (F₁) hybrid maize seed comprising crossing the plant of claim2 with a different inbred parent maize plant and harvesting theresultant first generation (F₁) hybrid maize seed.
 8. The method ofclaim 7 wherein inbred maize plant of claim 2 is the female or maleparent.
 9. An F₁ hybrid seed produced by crossing the inbred maize plantaccording to claim 2 with another, different maize plant.
 10. An F₁hybrid plant, or parts thereof, grown from the seed of claim
 9. 11. Aprocess for producing inbred PH05G comprising:planting a collection ofseed comprising seed of a hybrid, one of whose parents is Inbred PH05Gsaid collection also comprising seed of said inbred;growing plants fromsaid collection of seed; identifying said inbred plants; selecting saidinbred plant; and controlling pollination in a manner which preservesthe homozygosity of said inbred plant.
 12. The process of claim 11wherein said step of identifying said inbred plant comprises:identifyingplants with decreased vigor.
 13. The process of claim 11 wherein saidstep of identifying said inbred plant comprises:identifying seeds orplants with homozygous genotype.